rabbit polyclonal igg against egfr  (Santa Cruz Biotechnology)

Journal: Oncology letters

Article Title: Epidermal growth factor receptor expression and gene copy number analysis in gastric carcinoma samples from Chinese patients.

doi: 10.3892/ol.2015.3875

Figure Lengend Snippet: Figure 1. Immunohistochemical analysis of EGFR protein expression in GC. (A) GC exhibiting weak EGFR expression. (B) GC exhibiting EGFR overexpres sion (magnification, x200). EGFR, epidermal growth factor receptor; GC, gastric carcinoma.

Article Snippet: Immunohistochemical staining of samples was performed using rabbit polyclonal IgG against EGFR (anti‐EGFR; 1:50; sc03; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and avidin-biotin-peroxidase techniques (VECTASTAIN® Elite ABC kit; Vector Laboratories, Inc., Burlingame, CA, USA).

Techniques: Immunohistochemical staining, Expressing

Journal: Oncology letters

Article Title: Epidermal growth factor receptor expression and gene copy number analysis in gastric carcinoma samples from Chinese patients.

doi: 10.3892/ol.2015.3875

Figure Lengend Snippet: Figure 2. Fluorescence in situ hybridization analysis of EGFR gene copy number in GC. EGFR produced a red signal and chromosome 7 centromere produced a green signal; nuclei were stained by DAPI which appeared as a blue signal. (A) GC cells exhibiting gene amplification demonstrated a formation of clusters with numerous signals for EGFR. (B) GC cells exhibiting increased EGFR copy number due to chromosome 7 polysomy (magnification, x100). EGFR, epidermal growth factor receptor; GC, gastric carcinoma.

Article Snippet: Immunohistochemical staining of samples was performed using rabbit polyclonal IgG against EGFR (anti‐EGFR; 1:50; sc03; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and avidin-biotin-peroxidase techniques (VECTASTAIN® Elite ABC kit; Vector Laboratories, Inc., Burlingame, CA, USA).

Techniques: Fluorescence, In Situ Hybridization, Produced, Staining, Amplification

Journal: Oncology letters

Article Title: Epidermal growth factor receptor expression and gene copy number analysis in gastric carcinoma samples from Chinese patients.

doi: 10.3892/ol.2015.3875

Figure Lengend Snippet: Figure 3. Survival curves constructed using the Kaplan‑Meier method and log‑rank test. (A) Survival curves revealed that GC patients exhibiting weak EGFR expression (1+) and EGFR overexpression (2+) possessed an unfavorable prognosis compared with EGFR‑negative GC patients (0). (B) Survival curves revealed that GC patients demonstrating increased EGFR gene copy numbers, as detected by FISH, possessed an unfavorable prognosis. GC, gastric carcinoma; EGFR, epidermal growth factor receptor; FISH, fluorescence in situ hybridization; Cum, cumulative.

Article Snippet: Immunohistochemical staining of samples was performed using rabbit polyclonal IgG against EGFR (anti‐EGFR; 1:50; sc03; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and avidin-biotin-peroxidase techniques (VECTASTAIN® Elite ABC kit; Vector Laboratories, Inc., Burlingame, CA, USA).

Techniques: Construct, Expressing, Over Expression, Fluorescence, In Situ Hybridization

Journal: BioMed Research International

Article Title: Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

doi: 10.1155/2015/657179

Figure Lengend Snippet: Deguelin reduced the expression of phosphorylated IGF1R, p-Akt, and p-ERK and induced apoptosis in SCC-4 cell lines. Subconfluent culture was treated with deguelin at different concentrations for 24 h. Whole-cell extracts were prepared and analyzed by Western blot using antibodies against p-Akt, Akt, p-ERK, and ERK (a); p-EGFR, EGFR, p-IGF1R, and IGF1R (b); and PARP (c-PARP, cleaved PARP; u-PARP, uncleaved PARP; total PARP, sum of cleaved and uncleaved PARP) (c). Total cell extracts from Jurkat cells: serum starved overnight and then treated with Calyculin A was used as positive control (PC) for p-Akt and Akt.

Article Snippet: Mouse monoclonal antibody against phosphorylated-ERK1/2 (p-ERK1/2) and rabbit polyclonal antibody against total EGFR were from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Western Blot, Positive Control

Journal: BioMed Research International

Article Title: Deguelin Induces Apoptosis by Targeting Both EGFR-Akt and IGF1R-Akt Pathways in Head and Neck Squamous Cell Cancer Cell Lines

doi: 10.1155/2015/657179

Figure Lengend Snippet: Deguelin induced apoptosis by targeting both EGFR-Akt and IGF1R-Akt pathways in HNSCC cell lines. Subconfluent cultures were incubated for 24 h in serum-free medium. After the starvation, cells were treated with 10 μ M deguelin for 1 h. (a) The deguelin-treated SCC-4 cells were incubated for 15 min and 24 h with or without 10 ng/ml of IGF, respectively. (b) The deguelin-treated HSC-4 cells were incubated for 24 h with or without 10 ng/ml of EGF. Whole-cell extracts were analyzed by Western blot using antibodies against p-Akt, Akt, and PARP. (c) HSC-4 cells were treated with deguelin at different concentrations for 24 h in 10% FBS-containing medium. Whole-cell extracts were analyzed by Western blot using antibodies against p-EGFR, EGFR, and PARP (c-PARP, cleaved PARP; u-PARP, uncleaved PARP; total PARP, sum of cleaved and uncleaved PARP).

Article Snippet: Mouse monoclonal antibody against phosphorylated-ERK1/2 (p-ERK1/2) and rabbit polyclonal antibody against total EGFR were from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Incubation, Western Blot